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Data for membrane mobility of three different proteins

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Question 4: [8 pts];Data for membrane mobility of three different proteins (X, Y, and Z) using fluorescent recovery after;photobleaching (FRAP) are shown on the left in the figure below.;Then, the movement of each protein in the membrane is followed through single-particle tracking (SPT). The data;collected is shown on the right side of the image above.;a. [5pts] Assign an SPT profile (A, B, or C) to each of these proteins on the basis of the respective FRAP profiles.;Explain your choice.;b. [3pts] It is important to remember that in each of these experiments, the results reflect a real, physical;difference in the way in which these proteins are situated in/around the plasma membrane. Where do you think;each of these proteins is located? [No need to provide an explanation];Question 5: [12 pts];Extremophiles are microorganisms, usually from the Archeal domain that grow under a wide variety of extreme;conditions (temperature, pressure, etc). Some of these extremophiles can grow in temperatures up to 120C, the;sterilization temperature of an autoclave. Identifying the adaptations that allow these microorganisms to thrive at;such high temperatures is an area of active research both for improving industrial processes (i.e. biofuels) and;exobiology. Recent studies of the membrane composition have found that the major lipids in these organisms are;tetraether phospholipids and diether phospholipids. In the diagrams below, the R represents a variety of head;groups.;a. [3pts] Diagram what a membrane made up of diether phospholipids would look;like. Compare this to the eukaryotic cell membrane shown on the right.;Comparison should include: fluidity, transition temperature and structure.;b. [3pts] Diagram what a membrane made up of tetraether phospholipids would look like. Compare this to the;eukaryotic cell membrane shown in question a. Comparison should include: fluidity, transition temperature and;structure.;c. [3pts] Diagram what a membrane made up of tetraether and diether phospholipids would look like. Compare;this to the eukaryotic cell membrane shown in question a. Comparison should include: fluidity, transition;temperature and structure.;d. [3pts] Would you expect either of the phospholipids in the thermophiles membrane to become more or less;saturated as the temperature increases? Explain your answer.

 

Paper#15311 | Written in 18-Jul-2015

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