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Method for measuring enzyme activity

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Bio103OutlFP.docx Download Attachment;outline Final Applied Lab Project;Instructions;Outline (10 points) submit in this assignment folder by deadline.;See syllabus for detailed description and grading rubric for the final applied lab project;Include the following in your outline;Name of enzyme you will use;Name of organism (if applicable);The substrate;Method for measuring enzyme activity;Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples;The control(s) in the experiment;Sample size;Hypothesis;How you will present your data (table and/or type of graph);Anything else you would like to get feedback on before you start your experiment;----------------------------------------------------------------------------------------------------------------------------------Final Applied Lab Project;Instructions;Final Applied Lab Project (1 credit Lab Component);Addresses course outcomes 1-4:recognize and explain how the scientific method is used to solve problems;*make observations and discriminate between scientific and pseudoscientific explanations;weigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific;method;use knowledge of biological principles, the scientific method, and appropriate technologies to ask relevant;questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusions;This is the culminating lab assessment in BIOL 103. It is designed to assess your ability to apply the principles of the;scientific method.;The Effect of low pH on Enzyme Activity;Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are;proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, the OLI module;about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded t;impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as;treatment), or you can search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your;project (include all references).;As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will;modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the;question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design;you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the;acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your;also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment;as well as knowledge of enzymes and pH from the OLI modules, lab manual and potentially additional information;sources.;Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH;value could change the shape of the active site on the enzyme protein), without directly participating in the reaction.;Lab Materials;You may need all or some of the following, depending on your experimental design;Materials from your lab kit;pH paper;hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with;the lab kit);plastic beakers or cups;vinegar;yeast (can be purchased at grocery store if more is needed);balloons;plastic bottle;marker for labeling of beakers;You may choose to use additional materials (different acidic fluids and/or different organisms and/or different substrate;if you chose an enzyme other than catalase).;Outline (10 points) submit in assignment folder in week 7.;Include the following in your outline;Name of enzyme you will use;Name of organism (if applicable);The substrate;Method for measuring enzyme activity;Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples;The control(s) in the experiment;Sample size;Hypothesis;How you will present your data (table and/or type of graph);Anything else you would like to get feedback on before you start your experiment;Write a paper that includes the following;1. Title page: descriptive title, your name, course name, semester;2. Introduction: general background information about enzymes and specific information about your chosen enzyme;the question(s) that you are asking and a clear hypothesis for your experiment (15 points).;3Design an experiment. Provide a detailed description of the materials and methods used to conduct the experiment.;Identify control and experimental samples, as well as independent and dependent variables. Also include the methods;used for data collection and analysis (20 points).;4. Conduct the experiment and record your results. What did you observe? Present your data in table and/or graph format.;include the unit of measure with all numbers (20 points);5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to;the OLI course modules, lab manual and/or use additional information resources. What effect does the acidic treatment;have on enzyme activity? Did you get the expected results? Explain. (15 points);6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back, how could you;have improved your experiment? (10 points);7. Include a list of references to all information sources used in APA format (5 points).

 

Paper#15339 | Written in 18-Jul-2015

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