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RBFox-1 gene in heart disease

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I need homework help with my biomedical research class. Each question should be answered in half a page single spaced. The questions are about research done on the RBFox-1 gene in heart disease. For question number 2, the disease model used was the TAC model in mice. Please answer each question in a half a page each.;Attachment Preview;problem set #2-1.pdf Download Attachment;BR5HASummer2014ProblemSet2;Due:Tuesday,August19,2014;As promised, here is problem set #2. Note that the questions require logical thought and;imagination more than good memory and good note taking (although your notes will certainly;helpyougetideastoanswerthesequestions).Remember,therearenosinglerightanswers.Each;questionisworth12.5points,foratotalof50pointsonthisproblemset.;Pleasedonthesitatetocomebyofficehours,ormakeanappointmentwithmeifyoufeelthata;particularquestionisunclear.;Pleasereadtheseinstructionscarefully;Each question should be answered in no more than half a single spaced page. Therefore the;assignmentshouldnotexceedtwosinglespacedpages.;Dontusefontsmallerthan10pointsothatIcanreadwithoutstrainingmyeyes.;1) In class we discussed the importance of finding a biomarker that can reliably detect heart failure;especially beforemajorsymptomsbecomeobvioustothepatient. To answerthisquestion,imagine;thatanewbiomarkerforheartdiseaseisdiscovered.Youreadthatthisbiomarkerisaproteinwhich;isnormallynotfoundinblood,butwhichappearsin80%ofasymptomaticpeoplewhowilleventually;goontodevelopheartdisease.Thearticleyoureadisverypositiveaboutthisnewbiomarkerandits;potential to save lives, but being a critical thinker you decide to play the role of devils advocate. Is;thereanypotentialdisadvantageofusingthisbiomarker?Wouldyouultimately(afterconsideringthe;prosandcons)beinfavororagainstitsuse?Why?;2)HowdidDr.Wangmodelchronicheartdiseaseinmice?Howdoesthismodelcomparetoactualheart;disease in humans? What are some of the disadvantages of using this disease model? Why was this;diseasemodeluseddespiteitsflaws?;3) One of the surprising findings in this seminar is that the splicing patterns of diseased heart tissue;mirrorthesplicingpatternsofthedevelopingheart(seeslide15).Rememberthatwestilldontknow;exactly what this means, but this should not stop us from thinking about it. For example, we could;makeanargumentthatthesplicingpatternsweseeinthediseasedheartaredamaging(i.e.casing;theactualdamagetotheheart).Alternativelywecouldjustaseasilysaythatthesesplicingpatterns;are protective (i.e. helping minimize damage to the heart). Notice that these two statements are;startingtolooklikealternativehypotheses.So,whatwouldyouchooseasyourinitialhypothesis?Do;you think that the splicing patterns are damaging or protective? How would you justify your;choice? Last, conceptually how would you test your hypothesis? (Notice that I say conceptually, so;pleasedontfocusonexperimentaltechniques.Focusonthelogicofyourexperiment.);4) Takealookatthehypotheticalresultsseeninfigure1.HereyouseearepresentationofaqRTPCR;experimenttellingyouthattube#2(diseasedheart)containsmoreoftheheartstructuralgenethan;tube#1(healthyheart).Youknowthisbecausethesignalreachesthethresholdfirstintube#2.Ifyou;take a look at figure 2 you notice that this makes perfect sense as there is more heart structural;mRNA in tube #2 to begin with, so you might even feel safe to conclude that indeed the diseased;1;heart produces more of the heart structural mRNA and that this change in expression might be;associated with heart disease. Why would this conclusion be flawed? How can the housekeeping;genehelpyouprovetheflaw?;[Note:ahousekeepinggeneisoneofthemanygenesthatareexpressedmoreorlessequallyinallcells;andhelpsmaintainthem].

 

Paper#15347 | Written in 18-Jul-2015

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