44-0054-00-01 RPT Enzymes Temperature pH and Specificity.docx Download Attachment;Enzymes;Specificity;Temperature;pH;and;Hands-On Labs, Inc. Version 42-0054-00-01;b e r sitn;L R s s a t;a;po A;t;This document is not meant to be a substitute for a formal laboratory report. The Lab;Report Assistant is simply a summary of the experiments questions, diagrams if needed;and data tables that should be addressed in a formal lab report. The intent is to facilitate;students writing of lab reports by providing this information in an editable file which can be;sent to an instructor.;Exercise 1: Enzyme Specificity;sr a ns;Ob tio;e v;Data Table 1: Glucose Concentration;Wells;a;b;c;d;e;Concentration of Glucose;Qu sti ns;e o;A. What determines a persons ability to digest lactose?;B. Which of the wells showed a positive result for glucose? Explain the results.;C. Explain why testing for glucose is used to determine the activity of the enzyme;lactase.;D. Explain the experimental conditions for the five different wells.;able 2: Presence of glucose in wells indicating lactase activity at;various temperatures;Well;Time (min);Concentration of Glucose;5;a;10;15;20;5;b;10;15;20;5;c;10;15;20;Exercise 2: Enzymes and Temperature;sr a ns;Ob tio;e v;Qu sti ns;e o;A. Graph the effect of temperature on the activity of the enzyme lactase.;B. What happens when an enzyme is boiled? Is this effect reversible?;C. Based on your experiment results, what is the optimal temperature for lactase;function?;D. Explain what happens as far as the effectiveness of the enzyme at the freezing;temperature. Can this effect be overcome when the temperature rises?;Exercise 3: Enzymes and pH;sr a ns;Ob tio;e v;Data Table 3: Glucose in wells a-d indicates enzyme activity at various pH;levels;Well;a;b;c;d;pH;3.5;5.0;6.8;11.0;Concentration of Glucose;Qu sti ns;e o;A. Graph the data placing glucose concentrations on the y-axis and the pH values on;the x-axis.;B. What was the effect of pH on the enzyme lactase? Is this true for all enzymes?
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