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What is Genomics/

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Microarray worksheet (8 questions multiple choice);Attachment Preview;BS101_lab_6_Microarray_WORKSHEET (1).docx Download Attachment;Page 1 of 3;Lesson6Laboratory:Microarray;1.Genomicsisthestudyof;a.Thestructureandfunctionofmutationsandhowtheyaltergenetictraits.;b.GenesandtheDNAsequencesbetweengenesandhowtheydetermine;development.;c.TheinformationprovidedbycomputerprogramswhichanalyzesmRNA.;d.Thehumangenomeascomparedtoothervertebrategenomes.;2.Microarraysareaveryusefultoolingenomicsbecausethey;a.HelpscientistsexamineintergeneticDNAbyseparatingitfromgenes.;b.Provideauniquepromoterregionforpolymerasechainreactions.;c.Allowscientiststoexaminethousandsofgenesallatonce.;d.DecreasethetimeittakesforscientiststomakecopiesofDNA.;3.Generally,everycellinourbodycontainsthesame20,000(orso)genes.However,cellsin;ourbodyaredifferentfromeachotherbecausethey;a.Havedifferentgenesturnedonorofftosupportdifferentfunctions.;b.Containdifferentcopiesofgenesfordifferentfunctions.;c.Providedifferentnucleotidebasesforeachdevelopmentalfunction.;d.Functiondifferentlybasedonvaryingproteomics.;TYPETHECORRECT;ANSWERSHERE;(a,b,c,d);D;a;4.Howcanscientistsdeterminethefunctionofordifferencesbetweencelltypes?Theycan;examinethe;a.Numberofnucleotidebasesingenesversusintergeneticsequences.;b.AmountofmRNAexpressedforeachgeneinacelltype,andthencomparethatinformation;betweencelltypes.;c.Amountofmutationsbetweengenesintheintergeneticspaces.;d.NumberoftRNAcopiesforaparticularcelltype.;b;5.Howisamicroarrayconstructed?Ineachspot,thereare;a.Copiesofallthegenesforanorganism.;b.Multiplecopiesofonegene,eachspothascopiesforadifferentgene.;c.Multiplecopiesofintergeneticsequences,whichbindtogenesinthesamples.;d.Copiesofintergeneticsequences,whichpromotethereplicationofDNAinasample.;C;6.TheexperimentthatbeginsinChapter3ofthesimulationseekstoanswerthequestion;a.Whatisthedifferencebetweenintergeneticspacesincancercellsversushealthycells?;b.WhydodifferentcelltypesexpressdifferentamountsofmRNA?;c.Howdodifferentcancercellsproducedifferentmutations?;d.Whatisthedifferencebetweenhealthycellsandcancercells?;C;Page 2 of 3;A;7.Whycantdoctorsusecellappearancetodiagnosecancer?;a.Notallcancercellslookdifferentfromhealthycells.;b.Cancercellsaretoosmalltoexamineusingcellappearance.;c.Notallcancercellsareabletobebiopsiedfromthebody.;d.Cancercellschangeappearancewhentakenoutofthebody.;8.Intheexperiment,asolventisaddedtoeachcelltype(healthycellsandcancercells).After;thesampletubecontainingeachcelltypeismixedonthevortex,theRNAisseparatedfromthe;restofthesampleinacentrifuge.WhydoesDNAsettletothebottomofthetubeandRNA;doesnt?;a.RNAismuchlongerthanDNA.;b.RNAisattachedtoproteinsthathelpitstayinsolution.;c.DNAisattachedtobiomoleculesthatweighitdownandhelpitsettletothebottom.;d.DNAismuchlongerthanRNA.;9.WhatfeaturedoesmRNAhavethattRNAandrRNAdonot?mRNAalways;a.ContainsaGABAbox.;b.ContainsaTATAsequence.;c.EndswithaGtail.;d.EndswithapolyAtail.;10.HowdothebeadsinthecolumnseparatemRNAfromallotherRNA?Thebeadscontain;a.SequencesthatmagneticallyseparatethemRNA.;b.Agluelikesubstancederivedfromspiderwebs.;c.PolyTs.;d.AsequenceofuracilsthatbindtothePolyAtail.;11.AfteryouisolatemRNA,youhavetomakeaDNAcopy.WhycantwejustusemRNA?;a.DNAismuchmorestablethanmRNA.;b.Wehavetoaddafluorescentlabelthatwillallowustoseethesample.;c.mRNAwilleventuallytransformintotRNAmakingitunusable.;d.AandB;12.Scientistscallhybridizationthekeytomicroarrays.Hybridizationoccurswhen;a.TwocomplimentarystrandsofDNAfromdifferentsourcesbindtoeachother.;b.PolyAtailsbindtoPolyTs.;c.DifferentspeciesinterbreedandcreatenewDNAbasepairings.;d.TwostrandsofidenticalDNAbindwithoutusingthetraditionalnucleotidepairs.;13.Whenyouscanthemicroarrayinthescanner,thedatashowsomedarkspots.Whatdo;theserepresent?;a.TheDNAthathasbeenreplicatedinhealthycells.;Page 3 of 3;b.ThemRNAthatwaswashedawayinthewashingsolution.;c.TheDNAthatwasnottranscribedandexpressedinhealthycells.;d.ThemRNAthatwasnotboundbyOligodtailsinthebeads.;14.Whenyouscanthemicroarrayinthescanner,somespotsareyellowandrepresentplaces;wherethegenewasexpressedinbothhealthyandcancercells.Thesespotstellus;a.Wheretolookformutations.;b.WhereDNAhybridizedincancercells.;c.ThatDNAexpressiondidntchangeinthesegeneswhencanceroccurred.;d.Thatthemicroarraydidntworkinthesegenes.;15.Inourexample,gene6219mRNAismadeinbothhealthyandcancerouscells,however;proteinsareonlytranslatedfromthatmRNAinhealthycells.Microarrayanalysis;a.Showsusthisdefectbymakingyellowspots.;b.Cannotshowusthisdefect,whichisalimitationofthistypeofanalysis.;c.Showusthisdefectbymakingredspots.;d.Cannotshowusthisdefect,whichisabenefitofthistypeofanalysis.

 

Paper#15498 | Written in 18-Jul-2015

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