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DNA and Protein synthesis




DNA and Protein synthesis sample.docx Download Attachment;DNA;and;Synthesis;Protein;Hands-On Labs, Inc. Version 42-005100-01;b e r sitn;L R s s a t;a;po A;t;This document is not meant to be a substitute for a formal laboratory report. The Lab;Report Assistant is simply a summary of the experiments questions, diagrams if;needed, and data tables that should be addressed in a formal lab report. The intent is to;facilitate students writing of lab reports by providing this information in an editable file;which can be sent to an instructor.;Exercise 1: Modeling;DNA;The students must attach the DNA model to this lab;report.;Note which nucleotides form pairscytosine and guanine, and adenine and thymine;form pairs.;s ns;Qu tio;e;A. The following list indicates the bases on one strand of a DNA molecule. Write in the;names of the bases that could pair with each to make the complementary strand.;cytosine;adenine;cytosine;cytosine;adenine;guanine;thymine;guanine;B. What are the three components of a DNA nucleotide? How do these molecules;connect together?;C. What are the four bases found in DNA? Which of these bases pair together?;Exercise 2: The Globin Gene;r u;Po re;ce d;1. Complete the DNA molecule by writing a complementary strand.;Coding Strand;CGT CTC TTC GGA CAC;Complementary Strand;2.;Write the mRNA sequence that will be created in the process of transcription. The;DNA coding strand has the information for the gene, so the strand must be;transcribed. The relationships are slightly different for RNA, because RNA does not;have T, therefore, U should be substituted for T. To transcribe DNA to RNA, the;pairing relationship is A U, T A, C G, and G C, respectively.;Coding Strand;CGT CTC TTC GGA CAC;mRNA Strand;3. codon is recognized, the protein creation is terminated. a reference. Remember, when a stop;Translate the mRNA into amino acids. Use Table 1 as;mRNA Strand;Amino Acids Formed;Qu sti ns;e o;A. How many nucleotides would it take to construct the mRNA coding strand of the subunit of the hemoglobin A molecule?;B. How many nucleotides would it take to model the entire -subunit of the hemoglobin A;molecule?;C. Is the -subunit of the hemoglobin A molecule a complete DNA molecule;(chromosome) or part of one? Explain.;D. What would happen if one of the DNA nucleotides was deleted? What if the first;T was substituted for an A? Would a substitution always result in a change? Explain why;or why not.;E. Using your newly formed model of DNA from Exercise 1, write the coding strand;below. Use the coding strand to determine the mRNA strand and the amino acids;formed. Do this separately for Row 1 and Row 2 of your DNA model.;Data Table: Newly Formed Model of DNA from Exercise 1;ROW 1;ROW 2;Coding Strand;mRNA Strand;Amino Acids;Formed;F. Did the new DNA model form any two of the same amino acids?;G. Optional: Compare the amino acids that were formed in this experiment with;those of classmates who also performed this experiment. Were there many similarities?


Paper#15721 | Written in 18-Jul-2015

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