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##### Calculating sodium clearance

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9. Suppose your sodium meter reading is 80 mV. Using the standard curve (see last page), calculate your sodium clearance using the flow rate from question 2.;I got a flow rate of 6.45 mL/min for question 2.;Attachment Preview;NPB quiz.docx Download Attachment;Name;NPB 101L, Quiz 6;Instructions;You may use any written references you deem necessary (e.g. lab manual, textbooks);but you MAY NOT consult with other students (past or present), other TAs, professors;neighbors, relatives, pets, extraterrestrials, friends (real or imaginary), beings from;another plane of existence, etc.;Please show all work, including appropriate units, and box your answer to;receive full credit.;1. Your weight is _______ lbs, which is equal to _______ kg. The lab manual (pg 67);specifies how much you should drink based on your weight. Calculate the four;volumes. You only need to show details of the calculations for one of the;experimental solutions.;a. Volume of distilled water you should drink;b. Volume of isotonic saline you should drink;c. Volume of bicarbonate you should drink;d. Volume of fluid you should drink if you are the control;2. The last time you urinated was at 10:40 AM. It is now 1:15 PM. You completely void;your bladder, filling a one-liter bottle. What is your flow rate in mL/min?;3. Assume your flow rate is 2.75 mL/min. Estimate the creatinine concentration of your;urine. Use the estimates of GFR and plasma creatinine concentration given in the;lab manual (pg 70). Express your answer in mg/L.;4. In order to accurately measure creatinine concentration using a spectrophotometer;you must dilute your estimated creatinine concentration to 30 mg/L. Using the value;of estimated creatinine concentration from question 3, calculate the required dilution;factor in order to wind up with a urine creatinine concentration of 30 mg/L. Round;your answer UP to the next whole number.;1;Name;NPB 101L, Quiz 6;5. Assuming you start with 1 mL sample of urine, how much distilled water should you;add in order to obtain a dilution factor of 12?;6. Suppose your optical density reading from the spectrophotometer is 1.1. Assume the;standard curve to estimate the creatinine concentration of your DILUTED urine;sample results in the following equation;y = 0.1411x 0.0236;where;y is the spectrophotometer reading;x is the creatinine concentration;a. Calculate the creatinine concentration of your DILUTED urine sample.;b. Using the dilution factor calculated in question 4, find the creatinine;concentration of your UNDILUTED urine.;7. Using the undiluted urine creatinine concentration you calculated from question 6;and the flow rate from question 2, calculate your creatinine clearance.;8. Is the creatinine clearance rate you calculated in question 7 closed to the expected;normal value for a healthy adult? If not, what should it be approximately (include;units)?;9. Suppose your sodium meter reading is 80 mV. Using the standard curve (see last;page), calculate your sodium clearance using the flow rate from question 2.;10. At what times should you prepare your diluted urine samples for creatinine analysis?;2;Name;NPB 101L, Quiz 6;y = 25.927Ln(x) - 56.3;Na+ Meter #1;150;Voltage, (mV);100;50;0;-50;-100;1;10;100;1000;Concentration, (mEq/L);SPEC. A;y = 0.1411x - 0.0236;1.6;1.4;1.2;Spec Reading;1;0.8;0.6;0.4;0.2;0;0;2;4;6;-0.2;Concentration, (m g/dL);3;8;10;12

Paper#16355 | Written in 18-Jul-2015

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