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Given the following DNA template strand of reporter gene

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I need solutions for bio 362 exam III;Attachment Preview;Exam 3-2013.pdf Download Attachment;Bio362-2013;Exam III;Name;Question 1. (30 pts);Given the following DNA template strand of reporter gene (a gene used to report on mRNA and;protein expression);5-CTATACTCCTCCATGTCTTACGTGGTAGTGGTGGTAGTGTACATCAAAAAAAAA-3;a) Write the likely mRNA sequence of this gene when it is expressed in bacteria. (2 points);b) Write the anticodon sequence of all tRNAs that could decode this mRNA (without Inosine;modifications in the tRNAs). Please make sure to indicate the correct directionality of;the codons and anticodons. (4 points);c) What is the minimum number of tRNAs required to decode this mRNA? Explain your;answer. (2 points);1;Bio362-2013;Exam III;Name;d) Write the likely amino acids sequence of the protein produced from this gene (2 points);e) Which approach/technique will you use to detect this mRNA? Briefly explain how this;approach works and what material/reagents you will need. (2 points);f) Which approach/technique will you use to detect this protein? Briefly explain how this;approach works and what material/reagents you will need. (3 points);2;Bio362-2013;Exam III;Name;g) You have discovered a new viral protein called Snoop that is a sequence specific mRNA;modification enzyme. When you express Snoop in bacteria or eukaryotes, along with your;reporter gene (shown above), you notice that you are unable to detect the reporter mRNA or;its protein product.;i.;Explain why there is no detectable reporter mRNA or protein when Snoop is;expressed in bacterial. (5 points);ii.;Explain which genes you will delete to stabilize the reporter mRNA and protein to;the effects of Snoop expression in bacteria? (5 points);iii.;Explain why there is no detectable reporter mRNA or protein when Snoop is;expressed in eukaryotes. (5 points);3;Bio362-2013;Exam III;Name;Question 2. Please provide brief definitions for the following terms (no more than one or two;sentences (10 pts);a. Attenuation;b. RRF;c. tmRNA;d. SspB;e. Molecular mimicry (Name one example);4;Bio362-2013;Exam III;Name;Question 3. (4 points);Briefly describe how the rate of transcription by Pol II might influence the inclusion or;exclusion of an exon in the mature mRNA.;Question 4. (6 points);You were given a six residue synthetic DNA oligonucleotide and asked to test its effects on;in vitro (in a test tube) protein synthesis. You find that this oligonucleotide inhibits bacteria;protein synthesis but not eukaryotic protein synthesis.;a) Briefly describe why this oligonucleotide inhibits bacterial protein synthesis. (4;points);b) What is the likely sequence of this oligonucleotide? (2 points);5;Bio362-2013;Exam III;Name;Question 5. (10 points);a) Briefly describe why the first base of stop codons is always U (not A, G, or C) and;the third base always an A or a G?;b) Why can RF2 accommodate either an A or a G at position 2 (UAA and UGA)?;6;Bio362-2013;Exam III;Name;Question 6. (10 points);You are the lab supervisor and someone gave you two precious tubes of RNA. One tube;contained bacterial mRNA and the other charged-tRNAs. The next day you found out;that one of the student mixed the two samples together into one tube, a BIG NO, NO.;After a lot of screaming and shouting you calm down and decide to purify the bacterial;charged-tRNAs away from the mRNAs since you need both RNAs for your experiments.;Describe one simple way of achieving this purification.;7;Bio362-2013;Exam III;Name;Question 7. (10 Points);a. How can some organisms get away with having fewer than 20 synthetases, yet still;charge tRNAs with all 20 amino acids (5 points);b. You have discovered a new anti-viral drug that only affects viral protein synthesis;without affecting normal/general eukaryotic protein synthesis. In a major scientific;conference you described in a simple sentence, or two, how your new drug;inhibits viral protein synthesis: (5 points);8;Bio362-2013;Exam III;Name;Question 9. (15 points);You were asked by the Center for Disease Control to evaluate three mutant viruses that;infect eukaryotic cells. You infect three different cell cultures to examine the effect of each;virus on translation of the host and the viral RNAs;a. You find that Virus 1 produces an altered protease that degrades eIF2B. What are the;consequences of this cleavage on translation of the host and viral RNAs?;b. You find that Virus 2 produces an altered protease that degrades eIF4E. What are the;consequences of this cleavage on translation of the host and viral RNAs?;c. You find that Virus 3 produces an altered methyl transferase that methylates the 2-OH;group of mRNAs on selected A residues, without modifying the polyA tail or affecting its;addition. What are the consequences of this modification on the host and viral RNAs?;9;Bio362-2013;Exam III;Name;10

 

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