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Basic DNA extraction techniques by isolating and visualizing DNA from a banana

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The purpose of this assignment is to introduce you to basic DNA extraction techniques by isolating and visualizing DNA from a banana. Although this exercise will be completed in your home, it is theoretically the same method scientists use in a laboratory setting to extract DNA from cells.;Directions;Open the link to the document to find the experimental procedure and instructions for extracting DNA from a banana. Once completed, you will submit a photograph of your final result, along with the answers to several questions included in the file.;Follow the detailed directions below;First, read the full instructions in "Isolating DNA From a Banana," Isolating DNA From a Banana.docx;Gather your supplies, most of which you may have at home already, others of which are readily available at local grocery stores or pharmacies.;Collect the DNA. Take a photograph and answer the questions in the lab document.;Save the lab document in.rtf,.doc or.docx format. Name it as "Isolating DNA From a Banana_Your Last Name.;Submit the completed lab document via the link above (the title of this assignment) as an attachment.;Attachment Preview;Isolating DNA From a Banana(1).docx Download Attachment;Unit 3, Lab 2;DNA Extraction from a Banana;Introduction;As you are learning in Unit 3, DNA is the blueprint for life and resides in the cells of all living;things. In this experiment, you will extract DNA from a banana using a few basic ingredients;many of which you may already have around the house.;In order to extract the DNA from cells, the cell and nuclear membranes must first be broken open;to allow the release of DNA. In a laboratory setting, this is done by mixing cells with a specific;type of detergent, which breaks apart (or lyses) the cell and nuclear membranes. In this case, we;will use kitchen dish detergent, which is a close cousin to the detergent used in labs. In order to;then isolate the DNA away from cellular debris, salt and alcohol are used. DNA is polar, and;therefore readily dissolvable in water. Salt and alcohol, however, reduce the polarity of the;DNA, causing it to separate (or precipitate) away from the water and become visible to the naked;eye as long, milky-looking strands. We will use table salt and 70% isopropyl alcohol (also;known as rubbing alcohol) for this step.;Materials;Banana;Ziplock bag;Measuring spoons and cups;Ice-Cold Alcohol (70% Isopropyl Alcohol, or rubbing alcohol, stored in freezer for at least an;hour before the experiment);Water;Table Salt;Liquid Dishwashing Detergent;Coffee filter (paper towel can be substituted);Toothpick, wooden skewer or wooden coffee stirrer (optional);Instructions;1) Place a 1-2 inch section of banana into a ziplock bag. Using your fingers, knead and;squeeze the banana until it is a mushy consistency and no solid form remains.;2) Fill a measuring cup with c lukewarm or room temperature water and add 1 tsp salt.;Stir to dissolve most or all of the salt.;3) Add the salt water mixture to the banana, seal the bag, and shake gently to mix for 45;seconds to mix.;4) Add 1 tsp liquid dish soap and again shake the bag gently for 45 seconds. Shake or;agitate gently to prevent too much dish soap foam from accumulating.;5) Set a coffee filter (or paper towel) over the top of a clear, narrow glass container, (a;drinking glass will do), pushing the center of the coffee filter down into the container;slightly, making a well, and overlapping the edges over the sides. If necessary, use a;rubber band to hold the filter in place.;6) Pour the banana mixture through the filter until most liquid containing the released DNA;has passed through, trapping the banana in the filter. You may have to pour the liquid;several times, waiting for it to drip through each time before adding more. You may also;discard the coffee filter and get a new one as the original one fills with banana making it;more difficult for the liquid to flow through.;7) Tilt your glass container at an angle and very slowly pour the ice cold isopropyl alcohol;down the inside of the container so that it slowly forms a layer on top of the DNA;mixture. Continue to pour until the layer of alcohol is approximately 1-2 inches thick.;8) Observe the interface where the food mixture and alcohol meet for approximately 5-10;minutes. The DNA will begin to accumulate in the alcohol layer as a milky-white;stringy substance. You can gently swirl a toothpick or wooden skewer to observe the;clumps of DNA, doing your best to not disturb the interface. Allowing the experiment to;sit for longer (up to an hour) will allow for more DNA accumulation.;9) Take a picture of your results and upload it with your written assignment (below).;To Submit For a Grade;Please complete the following short answer questions and submit them for your grade, along;with a picture of your completed experiment.;Please describe the purpose for each of the following reagents (ingredients) in your experiment.;In other words, why are these necessary in the DNA extraction process? Please use complete;and descriptive sentences when formulating your answers.;Banana;Salt;Dish Detergent;Isopropyl Alcohol

 

Paper#17523 | Written in 18-Jul-2015

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