i need the same tutor,rviswanathan;Attachment Preview;LU9 PS.docx Download Attachment;Question 1;1.;The figure depicts an experiment conducted in the 1950s that was used to discover a key;component of the immune response to a virus. This experiment was performed by infecting nonimmune cells in culture with an inactive form of the influenza virus. After incubation with the;inactive virus, the cells and virus were removed and the culture media is transferred to a second;culture of cells. The media alone from the initial culture was able to prevent influenza virus;replication in the second culture. What factor in the immune response to viruses was identified in;this experiment?;[x];Answer;2 points;Question 2;1.;Many viruses produce proteins that evade the interferon response. Match the viral protein with;its mechanism of inhibiting the interferon response.;Answer;InfluenzaAnswer;A Pseudo substrate for PKR;NS1.;B. Activates cellular phosphatase that;Vaccinia;dephosphorylates eIF2a;virus;C. Binds dsRNA and prevents PKR activation;K3L;D Interferon receptor analog that bnds;HSV;ICP34.5;EBV;Bcrf1.;interferon and prevents activation of cellular;interferon receptor;E. Interleukin analog that inhibits production of;interferons;Vaccinia;Virus;B18R;2 points;Question 3;1.;Match the following pattern recognition receptors with the viral component they recognize;Answer;Toll-like;Receptor 3;Toll-like;Receptor 4;Toll-like;Receptor 7;Answer;A Viral glycoproteins.;B. Unmethylated CpG;DNA;C. ssRNA;D.;dsRNA;Rig-I;Toll-like;Receptor 9;2 points;Question 4;1.;Match the labled boxes in the figure below with the correct....;Answer;Answer;A RIG-I.;B. Mx;C. Interferon;Receptor;D NFkB.;E. TLR9;F.;G.;H.;I.;J.;TLR3;eIF2alpha;PKR;TRIM5-alpha;RNase L;TLR4;K.;L. TLR7;12 points;Question 5;1.;The data below is recent data that a colleague of mine generated who obviously has not taken BIOL 513. RNA;aptamers are short RNA oligonucleotides (~80 nucleotides) that can be selected with high affinity to a specific;protein. A RNA aptamer was identified that binds specifically to one of the viral X proteins and inhibits a;protein/protein interaction essential for replication of Virus X. The idea of the experiment below was to see if the;aptamer would inhibit replication of the viruses in cells by measuring the cytopathic effect. The colleague;transfected the virus X aptamer into cells. As he had hoped, the aptamer inhibits the virus cytopathic effect. As a;control, the colleague also transfected a random aptamer that does not bind to any of the viral proteins and should;not inhibit replication. To his surprise, the random aptamer is just as effective as the viral aptamer at inhibiting viral;replication. What did the colleague fail to take into account when designing this experiment (3-4 sentences max)?;Answer;PressTabtoenterthecontenteditor.Forthetoolbar,pressALT+F10(PC)orALT+FN+F10(Mac).
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