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Can someone help me with this lab on enzymes

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Can someone help me with this lab on enzymes?;Attachment Preview;Enzyme Lab.doc Download Attachment;Experiment;En z ym;es;Enzymes;Margaret Vorndam;M.S. Version 42-005300-01;Observations;Note: The observed well colors should be recorded according to the following symbols. Collect;data until the well plate yields an amber color. Then, discontinue testing that test tubes contents.;++ = Black or blue-black, Starch present;+;= Light Blue or Light blue-black, Some starch present;- = Amber, Orange, or Red, No starch present;Data Table 1: Effect of Temperature on Amylase Enzyme Conversion of Starch to Sugar;Time;minutes;Well/Test Tube 1;0C;Well/Test Tube 2;37C;Well/Test Tube 3;Boiling (100C at STP);0;5;10;15;20;25;30;Place test tubes that still contain starch in 37C water;bath. Continue timing for 30 minutes.;60;Add 1 cm of alpha-amylase solution to test tubes that still contain;starch. Place in 37C water bath. Continue timing for 30 minutes.;90;www.LabPaq.com;1;Hands-On Labs, Inc.;Experiment;En z ym;es;Note: The observed well colors should be recorded according to the following symbols. Collect;data until the well plate yields an amber color. Then, discontinue testing that test tubes contents.;++ = Black or blue-black, Starch present;+;= Light Blue or Light blue-black, Some starch present;= Amber, Orange or Red, No starch present;Data Table 2: The Effect of Concentration on Amylase Enzyme Conversion of Starch;to;Sugar;Time;minutes;Test Tube #1;Concentration of;Amylase;Fill in % per;Test tube;#1 example;0.5 cm 2;cm, or 1:4;= 25%;Test Tube;#2;Test Tube;#3;%;%;Test Tube Test Tube;#4;#5;%;%;0;5;10;15;20;25;30;If test tube(s) still contains starch, let sit for 30 minutes and retest.;60;www.LabPaq.com;1;Hands-On Labs, Inc.;Experiment;En z ym;es;Note: The observed well colors should be recorded according to the following symbols. Collect;data until the well plate yields an amber color. Then, discontinue testing that test tubes contents.;++ = Black or blue-black, Starch present;+;= Light Blue or Light blue-black, Some starch present;- = Amber, Orange or Red, No starch present;Data Table 3: The Effect of pH on Amylase Enzyme Conversion of Starch to Sugar;Time, in minutes;pH (#1 example);Student to fill in;0;Test Tube #1 Test Tube #2;Test Tube #3;Test Tube #4;pH 3.5;pH;pH;pH;5;10;15;20;25;30;If test tube(s) still contains starch, let sit for 30 minutes and retest.;60;www.LabPaq.com;1;Hands-On Labs, Inc.;Experiment;En z ym;es;Exercise 1: Enzymes and Temperature;Questions;A. Which color indicated that starch was still present in the test tube?;Which color indicated that the starch was no longer present?;B. When the starch disappeared as indicated by the color change, what happened to it?;C. What is the variable in this exercise?;What could be considered the control treatment?;D. Based on the results recorded in Data Table 1, which temperature(s) is/are optimal for enzyme conversion activity?;Which temperature facilitated the conversion first?;Second?;E. Did other temperatures facilitate enzyme activity at all?;Why or why not?;www.LabPaq.com;168;Hands-On Labs, Inc.;Experiment;En z ym;es;F.;Why was the water bath temperature of 37C chosen as the mid-range temperature?;Hint: What is the F equivalent of this C temperature?;G. Why was it important to immediately observe the color of the test tube contents/iodine in;the microplate well?;H. Why didnt you add the iodine indicator directly to the test tubes?;I.;What happened when you incubated the test tube(s) that still contained amylase/starch at;37C?;Did both test tubes indicate the disappearance of starch after the incubation?;If not, why?;www.LabPaq.com;169;Hands-On Labs, Inc.;Experiment;En z ym;es;J. What happened after you added 1 cm of alpha-amylase solution to the test tube(s) that still;contained starch, and incubated them at 37C?;Why did this occur after the second addition of amylase, but not before?;K. Construct a graph that presents your findings from Table 1 with time on the x-axis, and relative concentration of starch on the y-axis (++ is highest, +, or is lowest). Include the graph in;the report.;Discussion;A. Was the initial hypothesis supported or refuted? Explain.;B. What have you learned from this experiment?;C. What practical applications could this knowledge have?;www.LabPaq.com;170;Hands-On Labs, Inc.;Experiment;En z ym;es;Exercise 2: Enzymes and Substrate Concentration;Questions;A. What is the variable in this exercise?;B. Which test tube has the highest concentration of alpha-amylase in the starch?;C. How could this experiment be improved?;D. Construct a graph that presents your findings in Table 2 with time on the x-axis, and relative;concentration of starch on the y-axis (++ is highest, +, or is lowest). Use five different colors;to represent the concentration of amylase. Include it in the report.;Discussion;A. Was your initial hypothesis supported or refuted as to which test tube starch contents would;convert to sugar first?;B. What have you learned from this experiment?;www.LabPaq.com;171;Hands-On Labs, Inc.;Experiment;En z ym;es;C. What practical applications could this knowledge have?;D. Describe another way to do this experiment that would test the same concept.;Exercise 3: Enzymes and pH;Questions;A. What is the variable in this experiment?;B. In which test tube(s) did the amylase convert starch to sugar?;Why might you expect this outcome?;C. Did any test tubes NOT exhibit a color;change? Why?;www.LabPaq.com;171;Hands-On Labs, Inc.;Experiment;En z ym;es;Discussion;A. Did the results support or refute your hypothesis as to which test tube starch contents would;convert to sugar first?;L aboratory Summary;A. What other conditions that may affect the action of enzymes?;B. How might you propose to test this?;C. What have you learned from this laboratory?;D. What practical applications might this knowledge have?;Copyright 2008 by Margaret E. Vorndam, 1413 County Road 671, Rye, CO 81069;www.LabPaq.com;173;Hands-On Labs, Inc.

 

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