Biology;Lab 9: Cellular Respiration;Pre-Lab Questions;Why is cellular respiration necessary for living organisms?;Why is fermentation less effective than respiration?;What is the purpose of glycolysis?;How many ATP molecules are produced in aerobic respiration? How many ATP molecules are produced during fermentation and glycolysis?;Experiment 1: Fermentation by Yeast;Data Tables and Post-Lab Assessment;Table 1: Yeast Fermentation Data;Tube;Initial Gas Height (mm);Final Gas Height (mm);Net Change;Post-Lab Questions;Include your hypothesis from Step 1 here. Be sure to include at least one piece of scientific reasoning in your hypothesis to support your predictions.;Did you notice a difference in the rate of respiration between the various sugars? Did the artificial sugar provide a good starting material for fermentation?;Was anaerobic fermentation occurring? How do you know (use scientific reasoning)?;If you observed respiration, identify the gas that was produced. Suggest two methods you could use for positively identifying this gas.;Hypothesize why some of the sugar or sweetener solutions were not metabolized, while others were. Research the chemical formula of Equal? and Splenda? and explain how it would affect yeast respiration.;How do the results of this experiment relate to the role yeast plays in baking?;What would you expect to see if the yeast cell metabolism slowed down? How could this be done?;Indicate sources of error and suggest improvement (for example, what types of controls could be added?).;Experiment 2: Aerobic Respiration in Beans;Data Tables and Post-Lab Assessment;Table 2: Bromothymol Blue Color Change Over Time for Pinto Bean Trial;Time;Pre-Soaked Pinto Beans;Dry Pinto Beans;No Pinto Beans;0 min;30 min;60 min;90 min;120 min;150 min;180 min;24 hours;Table 3: Bromothymol Blue Color Change Over Time for Kidney Bean Trial;Time;Pre-Soaked Kidney Beans;Dry Kidney Beans;No Kidney Beans;0 min;30 min;60 min;90 min;120 min;150 min;180 min;24 hours;Post-Lab Questions;How did the color of the bromothymol blue solution in each beaker change over time in each condition?;What is the mechanism driving the bromothymol blue solution color change?;What can be inferred from the color change of the bromothymol blue solution?;What evidence do you have to prove that cellular respiration occurred in the beans? Explain your answer.;What are the controls in this experiment, and what variables do they eliminate? Why is it important to have a control for this experiment?;If this experiment were conducted at 0 ?C, what difference would you see in the rate of respiration? Why?;Would you expect to find carbon dioxide in your breath? Why?;What else could you incorporate into this experiment to verify that the gas is responsible for the color change? Design an experiment that shows the steps required.
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