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UMUC Lab 4 UMUC Lab 4 Enzymes




UMUC Lab 4;UMUC Lab 4 Enzymes;UMUC Biology 102/103;Lab 4: Enzymes;INSTRUCTIONS;? On your own and without assistance, complete this Lab4AnswerForm electronically and submit it via Assignments by the date listed on your Course Schedule (under Content - Syllabus). NOTE: The Assignment tab closes promptly @ 11:59 PM ET and will not be available even a minute past the deadline. Save your Lab4AnswerForm in the following format: LastName_Lab4 (e.g., Smith_Lab4).;? I will be posting feedback under a private Lab 4 Feedback group in the Discussions after I have graded all the labs. You will gain access to the private Lab 4 area if you submit a complete lab on time.;? You must submit your document in a Word (.doc or.docx) or Rich Text Format (.rtf) to receive credit. I cannot open Works or WordPerfect files.;Experiment 1: Enzymes in Food (15 pts);Table 1: Substance vs. Starch Presence;Substance Resulting Color Presence of Starch?;Positive Control: Starch;Negative Control: Student Must Select;Food Product;Food Product;Saliva;Post-Lab Questions;1. What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment?;2. What is the function of amylase? What does amylase do to starch?;3. Which of the foods that you tested contained amylase? Which did not? What experimental evidence supports your claim;4. Saliva does not contain amylase until babies are two months old. How could this affect an infant?s digestive requirements?;5. Digestive enzymes in the gut include proteases, which digest proteins. Why don?t these enzymes digest the stomach and small intestine, which are partially composed of protein?;Experiment 2: Effect of temperature on enzyme activity (15 pts);Table 2: Record the effect of temperature on the production of gas;Tube Temperature;(?C) Balloon Circumference;(Uninflated in cm);Balloon Circumference;(Inflated in cm);1 (cold);2 (room temp);3 (hot);Post-Lab Questions;Post-Lab Questions;1. What reaction is being catalyzed in this experiment?;2. What is the enzyme in this experiment? What is the substrate?;3. What is the independent variable in this experiment? What is the dependent variable?;4. How does the temperature affect enzyme function? Use evidence from your data to support your answer.;5. Draw a graph of balloon diameter vs. temperature. What is the correlation?;6. Is there a negative control in this experiment? If yes, identify the control. If no, suggest how you could revise the experiment to include a negative control.;7. In general, how would an increase in substrate alter enzyme activity? Draw a graph to illustrate this relationship.;8. Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?


Paper#31403 | Written in 18-Jul-2015

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