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Enzyme Kinetics




Question;In;an initial experiment an enzyme-catalyzed reaction was carried out with the;substrate concentration a thousand times greater than the Km for that substrate.;After 9 minutes, 1% of the substrate had been converted to product, and;the amount of product formed in the reaction mixture was 12mmol.;In;a second experiment twice as much substrate and one-third as much;enzyme were combined;(i);Calculate the rate of the reaction (mmol min-1) in;the initial reaction.;(ii);Predict the effect of doubling the substrate in the second experiment;and explain your prediction with reference to Michaelis-Menten kinetics.;(iii);Calculate how long it will take for 12mmol of product to be;formed in the second reaction.


Paper#62480 | Written in 18-Jul-2015

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