Description of this paper

Preparing a PCR reaction for the thermocycle

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solution


Question

Question;Watch Videos and Answer 4 QuestionsVideo1: http://www.youtube.com/watch?v=2KoLnIwoZKU&feature=relmfuVideo 2: http://www.youtube.com/watch?v=JRAA4C2OPwg&feature=watch_response1.When preparing a PCR reaction for the thermocycler, a researcher will add taq polymerase, DNA nucleotides, and a 5? and 3' primer, among other components. For each of the components listed here, please indicate their purpose in the reaction.2. What does PCR stand for and what is the general purpose?3. PCR is performed in a Thermocycler, where varying temperatures are applied for a number of cycles indicated by the researcher. Please list the temperatures mentioned in the video and the reason that each temperature is applied to the sample.4. When preparing PCR reactions, researchers often include a water control that contains all of the components except the DNA. Why might a researcher want to include this sample?

 

Paper#62609 | Written in 18-Jul-2015

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