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UMUC Biology 102/103 Lab Lab 4: Enzymes




Question;Your Full Name;UMUC Biology 102/103;Lab4:Enzymes;INSTRUCTIONS;?;On your own and without assistance, complete;thisLab;4AnswerSheet electronically;and submit it via theAssignments;Folder by the date listedintheCourse Schedule (underSyllabus).;?;To conduct your laboratory exercises, use the;Laboratory Manuallocated under Course Content.;Read the introduction and the directions for each exercise/experiment carefully;before completing the exercises/experiments and answering the questions.;?;Save your Lab4AnswerSheet in the following format: LastName_Lab4 (e.g., Smith_Lab4).;?;You should submit your documentas a Word (.doc or.docx) or Rich;Text Format (.rtf) file for best compatibility.;Pre-Lab Questions;1. How;could you test to see if an enzyme was completely saturated during an;experiment?;2. List;three conditions that would alter the activity of an enzyme. Be specific with;your explanation.;3. Take a;look around your house and identify household products that work by means of an;enzyme. Name the products, and indicate how you know they work with an enzyme.;Experiment 1: Enzymes In;Food;Data Tables and Post-Lab Assessment;Table 1: Substance;vs. Starch Presence;Substance;Resulting Color;Presence of;Starch?;Positive Control: Starch;Negative Control:Student Must Select;Food Product;Food Product;Saliva;Post-Lab Questions;1. What were your controls for this experiment? What did they;demonstrate? Why was saliva included in this experiment?;2. What is the function of amylase? What does amylase do to;starch?;3. Which of the foods that you tested contained amylase? Which;did not? What experimental evidence supports your claim?;4. Saliva does not contain amylase until babies are two months;old. How could this affect an infant?s digestive requirements?;5. There is another digestive enzyme (other than salivary;amylase) that is secreted by the salivary glands. Research to determine what;this enzyme is called. What substrate does it act on? Where in the body does it;become activated, and why?;6. Digestive enzymes in the gut include proteases, which;digest proteins. Why don?t these enzymes digest the stomach and small;intestine, which are partially composed of protein?;Experiment 2: Effect of;Temperature on Enzyme Activity;Data Tables and Post-Lab Assessment;Table 2: Balloon;Circumference vs. Temperature;Tube;Temperature (?C);Balloon;Circumference (Uninflated, cm);Balloon;Circumference (Inflated, cm);1 - (Cold);2 - (RT);3 - (Hot);Post-Lab Questions;1. What reaction is being catalyzed in this experiment?;2. What is the enzyme in this experiment? What is the;substrate?;3. What is the independent variable in this experiment? What;is the dependent variable?;4. How does the temperature affect enzyme function? Use;evidence from your data to support your answer.;5. Draw a graph of balloon diameter vs. temperature. What is;the correlation?;6. Is there a negative control in this experiment? If yes;identify the control. If no, suggest how you could revise the experiment to;include a negative control.;7. In general, how would an increase in substrate alter enzyme;activity? Draw a graph to illustrate this relationship.;8. Design an experiment to determine the optimal temperature;for enzyme function, complete with controls. Where would you find the enzymes;for this experiment? What substrate would you use?


Paper#62720 | Written in 18-Jul-2015

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