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UMUC Biology 102/103 Lab 2: The Chemistry of Life




Question;Your Full;Name;UMUC Biology 102/103;Lab 2: The Chemistry of Life;INSTRUCTIONS: ?;On your own and without assistance, complete;thisLab;2Answer Sheet electronically;and submit it via theAssignments;Folder by the date listed in theCourse Schedule (underSyllabus).?;To conduct your laboratory exercises, use the;Laboratory Manual located under Course Content. Read the introduction and the;directions for each exercise/experiment carefullybefore completing the;exercises/experiments and answering the questions. ?;Save your Lab 2Answer Sheet in the;following format: LastName_Lab2 (e.g.;Smith_Lab2).?;You should submit your document as a Word (.doc or.docx) or;Rich Text Format (.rtf) file for best compatibility.Pre-Lab Questions1. Nitrogen;fixation is a natural process by which inert or unreactive forms of nitrogen;are transformed into usable nitrogen. Why is this process important to life? (5;points)2. Given;what you have learned about the hydrogen bonding shared between nucleic acids;in DNA, which pair is more stable under increasing heat: adenine and thymine;or cytosine and guanine? Explain why. (5 points)3. Which;of the following is not an organic molecule: Methane (CH4);Fructose(C6H12O6), Rosane (C20H36);or Ammonia (NH3)? How do you know? (5 points) Experiment 1: Testing for ProteinsData Tables and Post-Lab AssessmentTable 1: A Priori Predictions;Sample;Will There Be Protein Present?;Initial Color;Final Color;1.;Albumin Solution;2.;Gelatin Solution;3.;Glucose;4.;Water;5.;Unknown;Sample;Initial Color;Final Color;Is Protein Present?;1.;Albumin Solution;2.;Gelatin Solution;3.;Glucose;4.;Water;5.;Unknown;Table 2: Testing for Proteins Results(15 points)Post-Lab Questions1. How;did your a priori predictions from Table 1 compare to your actual results in;Table 2? If there were any inconsistencies, explain why this occurred. (5;points)2. Identify;the positive and negative controls used in this experiment. Explain how each of;these controls are used, and why they are necessary to validate the;experimental results. (5 points)3. Identify;two regions in which proteins are vital components in the human body. Why are;they important to these regions? (5 points)4. Diet;and nutrition are closely linked to the study of biomolecules. Describe one;method by which you could monitor your food intake to ensure the cells in your;body have the materials necessary to function. (5 points);Experiment 2: Testing for Reducing SugarsData Tables and Post-Lab AssessmentTable 3:Testing for Reducing Sugars Results(15 points);Sample;Initial Color;Final Color;Reducing Sugar Present;1 - Potato;2 - Onion;3;- Glucose Solution;4;- Water;5;- Unknown;Post-Lab Questions1. Write;a statement to explain the molecular composition of the unknown solution based;on the results obtained during testing with each reagent. (5 points)2. What;can you conclude about the molecular make-up of potatoes and onions based on;the test you performed? Why might these foods contain these substance(s)? (5;points)3. What;results would you expect if you tested ribose, a monosaccharide, with;Benedict?s solution? Biuret solution? (5 points)Experiment 3: What Household Substances are Acidic or Basic?Data Tables and Post-Lab AssessmentTable 4:pH Values of Common Household Substances(5 points);Substance;pH Prediction;pH Test Strip Color;Acetic Acid;(Vinegar);Sodium;Bicarbonate Solution (Baking Soda);Post-Lab Questions1. What;is the purpose of determining the pH of the acetic acid and the sodium;bicarbonate solution before testing the other household substances? (5 points)2. Compare;and contrast acids and bases in terms of their H+ ion and OH-;ion concentrations. (5 points);3. Name;two acids and two bases you often use. (5 points)


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