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BIOL 102 Final Applied Lab Project - The Effect of low pH on Enzyme Activity (Use it as a Guide)




Question;Addresses course outcomes 1-4:? recognize and explain how the scientific method is used to solve problems? make observations and discriminate between scientific and pseudoscientific explanations? weigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific method? use knowledge of biological principles and the scientific method to ask relevant questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusionsThis is the culminating lab assessment in BIOL 102. It is designed to assess your ability to apply the principles of the scientific method as you design your own experiment.The Effect of low pH on Enzyme ActivityDesign an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: Enzymes are proteins.) It may be useful for you to review the Scientific Method Tutorial (found in the Course Content section of the classroom).It is acceptable to use one of the enzymes/substrates/methods from lab 4, or you can come up with your own based on a web-search. Be sure to cite the references if you use the web for ideas.Lab MaterialsYou may need all or some of the following, depending on your experimental design:Materials from your lab kit:? pH paper? hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit)? plastic beakers or cups? vinegar? yeast (can be purchased at grocery store if you need more)? balloons? plastic bottle? marker for labeling of beakersYou may choose to use additional materials (different acidic solutions and/or different organisms and/or different substrate(s) if you choose to look at an enzyme other than catalase).OutlineI suggest you begin by developing an outline that includes the following:? Name of enzyme you will use, and source (organism)? The substrate you will use? How you will measure the enzyme?s activity (method)? What type of treatment you will use, type of solution(s), pH, length of exposure, how you will treat your samples? The control(s) in the experiment? Sample size? How you plan to present your data (table and/or type of graph)AssignmentWrite a paper that includes the following:I. Title page:A. TitleB. Your nameC. Course name, semesterD. Instructor?s nameII. IntroductionA. Background informationB. Question(s) that you are askingC. Clear hypothesis for your experimentIII. Design and conduct the experimentA. Provide a detailed account of the materials and methodsB. Include the methods for data collectionC. Include the methods for data analysisIV. Data and ResultsA. List all observationsB. Present your data in table and/or graph (Remember to include the unit of measure.)V. ConclusionsA. Discuss the effect the acidic treatment had on the enzyme activityB. Clearly state your conclusion based on your experimental resultsC. Use your knowledge about enzymes and acids to interpret and discuss your results(It may be necessary for you to refer to your textbook and/or use other information resources.)D. Discuss how could you have improved your experimentE. State whether your original hypothesis was supportedVI. ReferencesA. Cite all references APA-styleB. Be sure to include in-text reference citationsVII. Grammar and spelling will be considered in grading your paper


Paper#62802 | Written in 18-Jul-2015

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