Question;Lab2;The Chemistry of Life;Pre-Lab Questions;1. Nitrogen fixation is a natural process by which inert or;unreactive forms of nitrogen are transformed into usable nitrogen. Why is this;process important to life?;2. Given what you have learned about the hydrogen bonding;shared between nucleic acids in DNA, which pair is more stable under increasing;heat: adenine and thymine, or cytosine and guanine? Explain why.;3. Which of the following is not an organic molecule, Methane;(CH4), Fructose(C6H12O6), Rosane (C20H36);or Ammonia (NH3)? How do you know?;Experiment 1: Testing;for Proteins;Data Tables and Post-Lab Assessment;Table 1: A Priori;Predictions;Sample;Initial Color;Final Color;Is Protein Present?;1. Albumin Solution;2. Gelatin Solution;3. Glucose;4. Water;5. Unknown;Sample;Initial Color;Final Color;Is Protein Present?;1. Albumin Solution;2. Gelatin Solution;3. Glucose;4. Water;5. Unknown;Table 2: Testing for;Proteins Results;Post-Lab;Questions;1. Write a statement to explain the molecular composition of;the unknown solution based on the results obtained during testing with each;reagent.;2. How did your a priori predictions from Table 1 compare to;your actual results in Table 2? If there were any inconsistencies, explain why;this occurred.;3. Identify the positive and negative controls used in this;experiment. Explain how each of these controls are used, and why they are;necessary to validate the experimental results.;4. Identify two regions which proteins are vital components in;the human body. Why are they important to these regions?;5. Diet and nutrition are closely linked to the study of;biomolecules. Describe one method by which you could monitor your food intake;to ensure the cells in your body have the materials necessary to function.;Experiment 2: Testing;for Reducing Sugars;Data Tables and Post-Lab Assessment;Table 3:Testing for Reducing;Sugars Results;Sample;Initial Color;Final Color;Reducing Sugar;Present;1 - Potato;2 - Onion;3 - Glucose Solution;4 - Water;5 - Unknown;Post-Lab Questions;1. What can you conclude about the molecular make-up of;potatoes and onions based on the test you performed? Why might these foods;contain these substance(s)?;2. What results would you expect if you tested ribose, a;monosaccharide, with Benedict?s solution? Biuret solution?;Experiment 3: What;Household Substances are Acidic or Basic?;Data Tables and Post-Lab Assessment;Table 4:pH Values of Common;Household Substances;Substance;pH Prediction;pH Test Strip;Color;Acetic Acid (Vinegar);Sodium Bicarbonate Solution (Baking Soda);Post-Lab Questions;1. What is the purpose of determining the pH of the acetic;acid and the sodium bicarbonate solution before testing the other household;substances?;2. Compare and contrast acids and bases in terms of their H+;ion and OH- ion concentrations.;3. Name two acids and two bases you often use.
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