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Final Applied Lab Project-BIOL 103

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Question;Final Applied Lab Project-BIOL 103The Effect of low pH on Enzyme ActivityDesign an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, the OLI module about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), but you are encouraged to search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references).As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the OLI modules, lab manual and potentially additional information sources.Submit Files - Final Applied Lab Project;Top of Form;Folder;Final Applied Lab Project;Instructions;Final;Applied Lab Project;Addresses;course outcomes 1-4;recognize and explain how the;scientific method is used to solve problems;make observations and;discriminate between scientific and pseudoscientific explanations;weigh evidence and make;decisions based on strengths and limitations of scientific knowledge and;the scientific method;use knowledge of biological;principles, the scientific method, and appropriate technologies to ask;relevant questions, develop hypotheses, design and conduct experiments;interpret results, and draw conclusions;This is;the culminating lab assessment in BIOL 103. It is designed to assess your;ability to apply the principles of the scientific method.;The;Effect of low pH on Enzyme Activity;Design;an experiment in which you will test the effect of an acidic fluid on;enzymatic activity. Recall: enzymes are proteins! To complete this project;it may be useful for you to first review the Scientific Method Tutorial, the;OLI module about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4;(Enzymes). As you review Lab 4, you will be reminded that there are several;factors that impact enzymatic activity: pH, temperature, and amount of;reagent. It is OK to use the same enzyme/substrate/method as you did in;lab 4 (but modify the treatment), but you are encouraged to search on-line to;find a different enzyme/substrate/method for measuring enzyme activity for;your project (include all references).;As you;design your experiment for this project, please remember that you are trying;to examine how an acidic fluid will modify the outcome of an;enzymatic reaction. To successfully complete this project, you will;need to identify the question(s) being asked in your experiment and the;hypothesis that you are testing. In your experimental design, you must;clearly explain what you are doing. That means that you will need to;identify the enzyme, the substrate, the acidic fluid used as treatment, the;control treatment and the method of measuring enzyme activity, as well as;explain your experimental protocol. You must also thoroughly explain;how the acidic fluid impacted enzyme activity based on the results from your;own experiment as well as knowledge of enzymes and pH from the OLI modules;lab manual and potentially additional information sources.;Hint: Keep in mind that the acid;will change the environmental conditions of the experiment (for example, a;low pH value could change the shape of the active site on the enzyme;protein), without directly participating in the reaction.;Lab;Materials;You may need;all or some of the following, depending on your experimental design;Materials;from your lab kit;pH paper;hydrogen peroxide solution;(you can purchase this at a pharmacy if you have used up the bottle that;came with the lab kit);plastic beakers or cups;vinegar;yeast (can be purchased at;grocery store if more is needed);balloons;plastic bottle;marker for labeling of;beakers;You may;choose to use additional materials (different acidic fluids and/or different;organisms and/or different substrate if you chose an enzyme other than;catalase).;Outline (10 points) submit in;assignment folder in week 7.;Name of enzyme you will use: Yeast;Name of organism (if;applicable): N/A;The substrate: Hydrogen peroxide;Method for measuring enzyme;activity: Circumference of balloons;Treatment: acidic fluid(s);pH, length of exposure, how you will treat your samples: length of exposure will be 1min, 3min, 5min;The control(s) in the;experiment: 3 Tubes filled with 10ml;of catalase, 10ml of hydrogen peroxide and no acetic acid;Sample size: 3 tubes with 10ml of catalase, 10ml of hydrogen;peroxide and each one with 1ml, 3ml, 5ml of acetic acid;Hypothesis: When acetic fluids are added to enzyme-substrate;mixtures, enzyme activity is affected.;How you will present your;data (table and/or type of graph);Table;Write a;lab report that includes the following;1. Title;page: descriptive title, your name, course name, semester;2. Introduction;general background information about enzymes and specific information about;your chosen enzyme, the question(s) that you are asking and a clear;hypothesis for your experiment (15 points).;3.;Design an experiment. Provide a detailed description of the materials and;methods used to conduct the experiment. Identify control and experimental;samples, as well as independent and dependent variables. Also include the;methods used for data collection and analysis (20 points).;4.;Conduct the experiment and record your results. What did you;observe? Present your data in table and/or graph format. Remember to;label everything and include the unit of measure with all numbers (20 points);5. Use;your knowledge of enzymes and pH to interpret and discuss your results. It;may be necessary for you to refer to the OLI course modules, lab manual;and/or use additional information resources. What effect does the acidic;treatment have on enzyme activity? Did you get the expected results?;Explain. (15 points);6. State;a specific and accurate conclusion. Is your hypothesis supported by the;results? Looking back, how could you have improved your experiment? (10;points);7.;Include a list of references to all information sources used in APA format (5;points).;Avoid;Plagiarism;It is;very important to write with your own words. If you do copy one;or two sentences directly (use sparingly), use quotation marks (");around the copied text, followed by an in-text reference. All information;sources need to be included in the reference list and as in-text references.;Plagiarism will be reported to the proper UMUC authority. Guidance on how to;avoid plagiarism can be found here;Submission;Submit;your final applied lab project to both the TurnItIn website as well as in the;assignment folder by the due date specified in the course schedule.;Grading;Your;Final Applied Lab Project will be graded based on the following criteria (100;points total);Rubric;Name: Final Applied Laboratory Project;Criteria;Excellent (90-100%);100 points;Good (75-89%);89 points;Adequate (50-74%);74 points;Unacceptable (0-49%);49 points;Outline 10 points (Due in week 7);Outline;includes all the required components and planned experiment meets;requirements for the assignment and is clearly and accurately described.;Submitted on time.;9-10;points;Outline;missing one or two of the required components, and/or planned;experiment does not meet one of requirements for the assignment and/or;minor issues with clarity and accuracy.;7-8;points;Outline;missing several of the required components, and/or planned;experiment does not meet several of the requirements for the assignment;and/or major issues with clarity and accuracy.;5-6;points;Outline;missing most of the required components andplanned experiment does;not meet the requirements of the assignment.;0-4;points;Introduction, Question and Hypothesis (15 points);Background;information about enzymes in general and about specific enzyme used in;project is clearly and accurately written. Questions and hypothesis are;specific, relevant and clearly stated.;14-15;points;Background;information about enzymes in general and about specific enzyme is somewhat;unclear and/or inaccurate. Questions and hypothesis could be more specific;relevant and clearly stated.;11-13;points;Missing;background information about enzymes in general or about specific;enzyme used in project or questions and hypothesis.;7-12;point;Missing;background information about enzymes in general and about specific;enzyme used in project andquestions/hypothesis.;0-6;points;Experimental Design, Materials and Methods (20 points);Experiment;is designed to directly test the hypothesis, description of experiment is;detailed and well written and includes all materials and methods used.;18-20;points;Experiment;is mostly designed to test the hypothesis and/or description of;experiment is somewhat inaccurate and/or some information about;materials and methods used is missing and/or minor problems with;clarity an organization.;15-17;points;Experiment;is barely designed to test the hypothesis and/or description of;experiment is inaccurate and/or most of the materials and methods;used are missing and/or major problems with clarity an organization.;10-14;points;Experiment;is not designed to test hypothesis and/or description of experiment;and materials and methods used are missing.;0-9;points;Results (20 points);Results;are clearly and accurately presented in a table and/or graph format.;18-20;points;Results;are presented, but minor problems with clarity and/or accuracy.;15-17;points;Results;are described, but major problems with clarity and/or accuracy and/or;results not presented in table or graph.;10-14;points;Results;are not included;0-9;points;Discussion of Results (15 points);A;clear, accurate and well organized discussion of results that demonstrates;good knowledge of enzymes.;14-15;points;Minor;problems with clarity, accuracy or organization and/or demonstrates;some gaps in knowledge of enzymes.;15-17;points;Major;problems with clarity, accuracy or organization or demonstrates;minimal knowledge of enzymes.;10-14;points;Missing;discussion of results, or major problems with clarity, accuracy or;organization andmissing understanding of enzymes.;0-9;points;Conclusion (10 points);A;well stated conclusion that is accurate and specific and directly related;to the data collected. Clearly stated whether or not the results support;the hypothesis. Relevant suggestion for improvement of experiment.;9-10;points;Conclusion;stated, but slightly inaccurate, and/or not directly related to data;collected and/or suggestion for improvement of experiment not very;relevant.;7-8;points;Conclusion;stated, but inaccurate and/or barely related to data collected and/or;missing suggestion for improvement of experiment.;5-6;points;Missing;conclusion, only suggestion for improvement of experiment.;0-4;points;Citations (5 points);All;references used are included and in correct APA format, in-text references;are included.;5;points;All;references are included, but a few minor mistakes in APA format, and/or;missing in-text references.;4;points;Missing;one or several references and/or major mistakes in APA format.;3;point;No;references included or references not in APA format.;0-2;points;Grammar and Spelling (5 points);Less;than 3 minor spelling and grammatical errors;5;points;Between;4 and 7 minor spelling and grammatical errors;4;points;Between;8 and 12 spelling and grammatical errors;3;point;More;than 13 spelling and grammatical errors;0-2;points;Overall Score;Level 4;90 or more;Level 3;75 or more;Level 2;50 or more;Level 1;0 or more;Bottom of Form

 

Paper#62829 | Written in 18-Jul-2015

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